| Enzyme | Function |
|---|---|
| DNA helicase | Unwinds and separates the two DNA strands |
| DNA polymerase | Adds complementary nucleotides to growing strand in 5' to 3' direction |
| RNA primase | Synthesizes RNA primers complementary to DNA strand |
| DNA ligase | Links DNA fragments by forming phosphodiester bonds |
| Topoisomerase | Prevents supercoiling ahead of replication fork |
When you use it
Use this table to review the specific functions of enzymes acting at the replication fork during the S phase.
Watch out
DNA polymerase cannot initiate synthesis on its own: it requires an RNA primer made by RNA primase and only extends from 5' to 3'.
Drafted from Grade 11 Biology, pages 113-179, then checked twice before it went up

